Generator

Part:BBa_K1448008:Design

Designed by: yuma kobayashi   Group: iGEM14_KIT-Kyoto   (2014-10-16)


In E coli mono-terpene production cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

How to use First, digest this part with SpeI Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI. Third, mix and ligate both samples.



Source

BBa_K1448007 from our design part. IPTG inducible tac promoter from pGEX-6P-2

References